Effect of Different Diluters on The Cooled Camel Semen Quality

Document Type : Original Research Articles (Regular Papers)

Authors

1 Animal Production Research Institute Agricultural Research Center

2 Animal Production l Department, Faculty of Agriculture, zagazig University

3 Department of Animal Production, Faculty of Agriculture, zagazig University

4 Animal Production development, Faculty of Agriculture, zagazig University

5 Animal production research institute

Abstract

The current study sought to determine how several extenders (Tris-Yolk-Fructose: TYF, Glucose-Yolk-Citrate: GYC, Fructose Yolk-Citrate: FYC, and Lactose-Yolk-Citrate: LYC) affected the enzymatic activity and quality of Maghrebi camel semen while stored at 5ºC for up to three days. During four hours of incubation at 37ºC, sperm penetration into she-camel cervical mucus was seen for various extenders. As compared to GYC and FYC extenders, the percentage of motile camel spermatozoa extended with TYF or LYC extenders was significantly higher (P<0.01), while the percentages of dead, abnormal, acrosome, and chromatin damage spermatozoa showed significantly (P<0.01) decreased when stored at 5°C for up to three days. The proportion of dead, aberrant, acrosome, and chromatin damage in camel spermatozoa extended with various extenders increased significantly (P<0.01) with storage at 5°C for up to three days, whereas the percentage of motile spermatozoa dropped. GYC and FYC extenders significantly (P<0.01) increased the activity of seminal Aspartate aminotransferase (AST) or Alanine aminotransferase (ALT) enzymes released into the extracellular medium of the male camel's semen when compared to T YF and LYC extenders during storage at 5°C for up to three days. The quantity of seminal AST and ALT enzyme activity released into the extracellular media of the male camel spermatozoa extended with various extenders increased significantly (P<0.01) with the increase in storage durations at 5°C for up to 4 days. The penetrating ability of camel spermatozoa was highly significantly with camel semen extended with LTC extender. We may ultimately conclude that TYF and LYC extenders are more suited for diluting camel semen when it is being stored at 5°C for up to three days than GYC and LYC extenders. The extended cooled spermatozoa with LYC extender produced the greatest (P<0.01) value of the camel spermatozoa's penetrating capacity into she-camel spermatozoa.  

Keywords

Files

Share

How to cite

Statistics