Impact of Various Serum Concentrations and Cryoprotectants on The in vitro Maturation of Vitrified Dromedary Camel Oocytes

Document Type : Original Research Articles (Regular Papers)

Authors

1 Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls).

2 Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt

Abstract

This study aimed to investigate the effects of two different doses (10% and 20%) of fetal bovine serum (FBS) with various cryo-protectant additives (CPAs; Ethylene glycol (EG) and Dimethyl sulfoxide (DEMSO)) on the maturation rate of dromedary camel oocytes. A total number of 2515 cumulus-oocyte complexes (COCs) were divided into seven experimental groups. As a control group (G1), the first group was rinsed with an in vitro maturation (IVM) medium and allowed to incubate for 30 hours. The remaining COCs were divided into six groups and treated with varying concentrations of vitrification solution (VS). First, COCs were transferred to an equilibration solution (ES) for two minutes. Afterward, the experimental groups were given 45 seconds in various VSs. Five COCs were put into 1-2 μl VS and loaded into open-pulled straws (OPS). The straws were immediately submerged in LN2 (-196 oC) for one hour. Following warming, the percentage of morphologically damaged oocytes revealed that G2 had the highest percentage (67.39%) and G6 had the lowest percentage (23.46%). The G1–G7 groups had a maturation rate of 78.24% 30.49%, 48.61%, 39.33%, 56.25%, 50.36%, and 68.75%, respectively, due to the expansion of cumulus cells. For the G1–G7 groups, the polar body extrusion results were 22.35%, 0%, 5.56%, 0%, 11.25%, 8.63%, and 6.25%, in that order. In conclusion, 20% FBS and DMSO as a single combination (G5) or combined with EG (G7) were the best methods for vitrifying dromedary camel oocytes.

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